何玉财 博士/教授/硕导

何玉财：男，博士，教授、硕导，主要从事生物质能源、生物基产品与生物催化合成研究。

GROUP MEMBERS（课题组成员）：
 2012届硕士研究生：周琼（联合培养）；

2013届硕士研究生：夏东琴（硕士研究生国奖获得者）（联合培养）；

2014届硕士研究生：杨振兴，龚磊，潘雪鹤（联合培养）；

2013级硕士研究生：陶志成（硕士研究生国奖获得者），张丹平（扬巴奖学金获得者）；

2014级硕士研究生：刘峰（硕士研究生国奖获得者，两次），丁云；

2015级硕士研究生：种刚刚；

2016级硕士研究生：姜春霞（硕士研究生国奖获得者，两次），薛新霞（硕士研究生国奖获得者），王冰倩，顾婷

2017级硕士研究生：黄晓君，张瑞钦；

2018级硕士研究生：李园园，朱旋，阳东

RESEARCH EXPERIENCE（研究兴趣）：

1）Bioconversion of cellulosic materials tofuel ethanol and other value-added products and chemicals

Specific Research Emphasis: a).Pretreatment of cellulosic materials and related reaction system design andkinetics study; b). Investigation of the synergetic effects between cellulaseand hemicellulase during enzymatic hydrolysis and its mechanics; c). Fermentation of sugars for production of bioethanol and other fuels; d). Investigationof the relationship between pretreatment and enzymatic hydrolysis and methodsto improve the overall cellulose and hemicelluloses conversion; e). Screeningof cellulase-producing strain and its fermentation optimization.

Biocatalysis

2） Biosynthesis of optically pure chiralcompounds or other important intermediates with extremely high values forpharmaceuticals and agrochemicals

Specific Research Emphasis: a).Screening and development of a lot of highly enantioselelctive biocatalysts bysubstrate-oriented screening, such as nitrilases, esterases and oxidoreductases;b). For production of highly value-added chiral synthons or otherintermediates, many efficient biotransformation processes, either by manipulatingthe medium composition (e.g., addition of organic cosolvents, metal ions,inducers, or surfactants like Tween-80) and the reaction conditions, or by optimizingthe bioreactor configuration or its operation mode, resulting in significant enhancementof selectivity and productivity; c). Immobilization technology is used for enhancing operation stability; d). Clone and overexpression of nitrilase and reductase.

ACHIEVEMENTS (成果)：

已发表SCI文章70余篇（其中在Green Chemistry、Bioresource Technology和Industrial Crops and Products 等杂志发表JCR一区26篇，在ACS Sustainable Chemistry & Engineering、Applied Microbiology and Biotechnology、Carbohydrate Polymers、Journal of Biotechnology和Journal of Industrial Microbiology and Biotechnology等杂志发表JCR二区12篇，在Applied Biochemistry and Biotechnology、Bioprocess and Biosystems Engineering、Biotechnology and Bioprocess Engineering、Bioengineered、Biotechnology Letters、Biology & Environment: Proceedings of the Royal Irish Academy、Chemical Papers、Catalysis Communications、Chinese Chemical Letters、Desalination and Water Treatment、Journal of the American Oil Chemists、Journal of Molecular Catalysis B: Enzymatic、Process Biochemistry等杂志发表30余篇），发表EI收录的文章11篇，申请中国专利近25项；引用次数1600次以上。

主要发表的JCR一区和二区的SCI文章：

JCR一区：（26篇）SCI

1. One-pot chemo-enzymatic conversion of D-xylose to furfuralcohol by sequential dehydration with oxalic acid plus tin-based solid acid and bioreduction with whole-cells. Bioresource Technology, 2018, 268: 292-299.

2. One-pot co-catalysis of corncob with dilute hydrochloric acid and tin-based solid acid for the enhancement of furfural production. Bioresource Technology, 2018, 268: 315-322.

3. Chemo-enzymatic synthesis of furfuralcohol from chestnut shell hydrolysate by a sequential acid-catalyzed dehydration under microwave and Escherichia coli CCZU-Y10 whole-cells conversion.Bioresource Technology, 2018, 262: 52-58.

4. Microbial lipid production from enzymatic hydrolysate of corn stover pretreated by combining with biological pretreatment and alkalic salt soaking. Industrial Crops and Products, 2018, 124: 487-494.

5. Enhanced bioreduction synthesis of ethyl (R)-4-chloro-3-hydroybutanoate by alkalic salt pretreatment. Bioresource Technology, 2018, 261: 196-205.

6. Biological synthesis of 2,5-bis(hydroxymethyl)furan from biomass-derived 5-hydroxymethylfurfural by E. coli CCZU-K14 whole cells.Bioresource Technology, 2018, 247: 1215-1220

7. One-pot conversion of biomass-derived xylose to furfuralcohol by a chemo-enzymatic sequential acid-catalyzed dehydration and bioreduction. Green Chemistry. 2017, 19: 3844–3850.

8. One-pot chemo-enzymatic synthesis of furfuralcohol from xylose. Bioresource Technology 2017, 238: 698-705.

9. Effective enzymatic in situ saccharification of bamboo shoot shell pretreated by dilute alkalic salts sodium hypochlorite/sodium sulfide pretreatment under the autoclave system. Bioresource Technology 2017, 241: 726-734.

10. Chemical-enzymatic conversion of corncob-derived xylose to furfuralcohol by the tandem catalysis with SO₄^2-/SnO₂-Kaoline and E. coli CCZU-T15 cells in toluene–water media. Bioresource Technology 2017, 245: 841-849.

11. Biological conversion of the aqueous wastes from hydrothermal liquefaction of algae and pine wood by Rhodococci. Bioresource Technology 2017, 224: 457-464.

12. Improving enzymatic saccharification of bamboo shoot shell by alkali salt pretreatment with H₂O₂. Bioresource Technology 2016, 201: 230-236.

13. A combined sodium phosphate and sodium sulfide pretreatment for enhanced enzymatic digestibility and delignification of corn stover. Bioresource Technology 2016, 218: 209-216.

14. Effective asymmetric bioreduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate by recombinant E. coli CCZU-A13 in [Bmim]PF₆–hydrolyzate media. Bioresource Technology 2016, 214: 414-418.

15. Effective pretreatment of sugarcane bagasse with combination pretreatment and its hydrolyzates as reaction media for the biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by whole cells of E. coli CCZU-K14. Bioresource Technology 2016, 211: 720-726.

16. Enzymatic in situ saccharification of chestnut shell with high ionic liquid-tolerant cellulases from Galactomyces sp. CCZU11-1 in a biocompatible ionic liquid-cellulase media. Bioresource Technology 2016, 201: 133-139.

17.Effective enzymatic saccharification of dilute NaOH extraction of chestnut shell pretreated by acidified aqueous ethylene glycol media. Industrial Crops and Products 2016, 81: 129-138

18. Significantly improving enzymatic saccharification of high crystallinity index’s corn stover by combining ionic liquid [Bmim]Cl–HCl–water media with dilute NaOH pretreatment. Bioresource Technology 2015, 189: 421-425.

19. Improved biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by adding L-glutamine plus glycine instead of NAD⁺ in β-cyclodextrin−water system. Bioresource Technology 2015, 182: 98-102.

20. Enhancement of enzymatic saccharification of corn stover with sequential Fenton pretreatment and dilute NaOH extraction. Bioresource Technology 2015, 193: 324-330.

21. Discovery of a reductase-producing strain recombinant E. coli CCZU-A13 using colorimetric screening and its whole cell-catalyzed biosynthesis of ethyl (R)-4-chloro-3-hydroxybutanoate. Bioresource Technology, 2014, 172: 342-348.

22. Highly efficient synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate and its derivatives by a robust NADH-dependent reductase from E. coli CCZU-K14. Bioresource Technology, 2014, 161: 461-464.

23. Enzymatic saccharification of sugarcane baggage by N-methylmorpholine-N-oxide-tolerant cellulase from a newly isolated Galactomyces sp. CCZU11-1. Bioresource Technology, 2013, 135: 18-22.

24. Biosynthesis of benzoylformic acid from benzoyl cyanide by a newly isolated Rhodococcus sp. CCZU10-1 in toluene-water biphasic system. Bioresource Technology, 2012, 115: 88-95.

25. Improving enzymatic hydrolysis of wheat straw using ionic liquid 1-ethyl-3-methyl imidazolium diethyl phosphate pretreatment. Bioresource Technology, 2010, 100(14): 3570-3575.

26. A Bronsted acidic ionic liquid as an efficient and environmental benign catalyst for biodiesel synthesis from free fatty acids and achohols. Bioresource Technology, 2009, 100(19): 4368-4373.

JCR二区:（12篇）SCI

27. Lipid production from dilute alkali corn stover lignin by Rhodococcus strains. ACS Sustainable Chem. Eng., 2017, 5: 2302-2311.

28. Enzymatic in situ saccharification of sugarcane bagasse pretreated with low loading of alkalic salts Na₂SO₃/Na₃PO₄ by autoclaving.Journal of Biotechnology 2017, 259: 73-82.

29. Efficient biosynthesis of rare natural product scopolamine using E. coli cells expressing a S14P/K97A mutant of hyoscyamine 6β-hydroxylase AaH6H.Journal of Biotechnology 2015, 211, 123-129.

30. Effective biosynthesis of ethyl (R)-4-chloro-3-hydroxybutanoate by supplementation of L-glutamine, D-xylose and β-cyclodextrin in n-butyl acetate–water media. Journal of Biotechnology 2015,203: 62-67.

31. Removal of Cu²⁺ from aqueous solutions by the novel modified bagasse pulp cellulose: Kinetics, isotherm and mechanism. Carbohydrate Polymers 2015, 129: 115-126.

32. Investigation of a novel acid catalyzed ionic liquid pretreatment method to improve biomass enzymatic hydrolysis conversion. Applied Microbiology and Biotechnology, 2014, 98: 5275-5286.

33. Highly enantioselective oxidation of phenyl methyl sulfide and its derivatives into optically pure (S)-sulfoxides with Rhodococcus sp. CCZU10-1 in an n-octane-water biphasic system. Applied Microbiology and Biotechnology, 2013, 97: 10329-10337.

34. Highly enantioselective oxidation of racemic phenyl-1,2-ethanediol to optically pure (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1. Applied Microbiology and Biotechnology, 2013, 97: 7185-7194.

35. Degradation of the novel herbicide ZJ0273 by Amycolatopsis sp. M3-1 isolated from soil. Applied Microbiology and Biotechnology, 2012, 96(5), 1371-1379.

36. A high-throughput screening strategy for nitrile-hydrolyzing enzymes based on ferric hydroxamate spectrophotometry. Applied Microbiology and Biotechnology, 2011, 89: 817-823.

37. Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system. Journal of Biotechnology, 2011, 152: 24-29.

38. Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by cetyl-trimethylammoniumbromide-permeabilized cells of Alcaligenes faecalis ECU0401. Journal of Industrial Microbiology and Biotechnology, 2010, 377: 741-750.